05). Translational motion increased significantly in high grade of disc and facet joint (except grade V disc and grade 4 facet joint) and with LFH in L1-L5 (P < 0.05). Angular motion significantly decreased in grade V disc, grade 4 ISL, and without LFH in L1-L5 (P < 0.05). According to muscle fatty degeneration,

translational and angular motions were not significantly changed.\n\nConclusion. Our results support that facet joint degeneration is followed by disc degeneration according to age. Increased translational movements of the lumbar segments occurred in severe disc degeneration accompanied by facet joint degeneration or the presence of LFH even if the movements were stabilized in the advanced status. Therefore, the current status of the intervertebral discs, facet joints, and ligamentum flavum should be taken into consideration when evaluating stability within the lumbar spine.”
“Objective: To analyze the influence C59 Wnt molecular weight of stapes prosthesis diameter on postoperative hearing results Napabucasin in vitro after stapedotomy without interposition in otosclerotic patients.\n\nData Sources: PubMed search from 1970 to 2009 using the key words stapedotomy or stapedectomy or otosclerosis or stapesplasty.\n\nStudy Selection: Inclusion criteria to select articles and patient groups for meta-analysis

and statistical analyses were as follows: otosclerosis as diagnosis, clear description of technique and prosthesis size, calibrated stapedotomy,

and complete report of functional results.\n\nData Extraction: Five controlled studies were found analyzing the influence Selleck Stem Cell Compound Library of prosthesis diameter and reporting the results in a comparable way for meta-analysis (n = 590). Sixty-two studies not analyzing the influence of prosthesis diameter contained comparable subgroups with a total of 9,536 cases. These cases were pooled according to their diameter (0.3, 0.4, 0.5, 0.6, and 0.8 mm). The results of air conduction, bone conduction, air-bone gap (ABG), and success rate (closure of the ABG within 10 dB as percentage of the total cases) for all groups and frequency-specific ABG results were gathered. Furthermore, 12 clinical and experimental studies were reviewed that did not contribute to the statistical analysis.\n\nData Synthesis: A meta-analysis performed for success rate of the 5 controlled studies showed favorable results for 0.6-mm over 0.4-mm prostheses (success rate, 67% versus 58%, p = 0.05). In the statistical analysis of the pooled data, the 0.6-mm prosthesis showed better results compared with 0.4 mm (p G 0.001) in the postoperative air conduction threshold (29 dB versus 35 dB), postoperative ABG (7 dB versus 11 dB), ABG improvement (25 dB versus 21 dB), and success rate (81.1% versus 75.1%). The frequency-specific analysis of the postoperative ABG showed no advantage for the small prosthesis in the high frequencies.

The hypothalamic paraventricular nucleus (PVN) is responsive to hypoxic stress and also contains neurons that express NMDA receptors and neuronal nitric oxide synthase (nNOS). We tested the hypothesis that extended (35 d) CIH results in a decrease in the surface/synaptic availability of the essential NMDA NR1 subunit in nNOS-containing neurons and NMDA-induced NO production in the PVN of

mice. As compared with controls, the 35 d CIH-exposed mice showed a significant increase in blood pressure and an increased density of NR1 immunogold particles located in the cytoplasm of nNOS-containing dendrites. Neither of these between-group differences was seen after 14 d, even though there was already a reduction GW4869 datasheet in the NR1 plasmalemmal density at this time point. Patch-clamp recording of PVN neurons in slices showed a significant reduction in NMDA currents after either 14 or 35 d exposure

to CIH compared with sham controls. In contrast, NO production, as measured by the NO-sensitive fluorescent dye 4-amino-5-methylamino-2′,7′-difluorofluorescein, was suppressed only in the 35 d CIH group. We conclude that CIH produces a reduction in the surface/synaptic targeting of NR1 in nNOS neurons and decreases NMDA receptor-mediated currents in the PVN before the emergence of hypertension, the development of which may be enabled by Fosbretabulin suppression of NO signaling in this brain region.”
“Phosphatase and tensin homolog on chromosome ten (PTEN) is

a tumor suppressor and an antagonist of the phosphoinositide-3 kinase (PI3K) pathway. We identified a 576-amino acid translational variant of PTEN, termed PTEN-Long, that arises from an alternative translation start site 519 base pairs upstream of the ATG initiation sequence, adding 173 N-terminal amino acids to the normal PTEN open reading frame. selleck compound library PTEN-Long is a membrane-permeable lipid phosphatase that is secreted from cells and can enter other cells. As an exogenous agent, PTEN-Long antagonized PI3K signaling and induced tumor cell death in vitro and in vivo. By providing a means to restore a functional tumor-suppressor protein to tumor cells, PTEN-Long may have therapeutic uses.”
“Well defined, stable, one-point binding ruthenium complexes 1 and 2 selectively bind and activate alpha,beta-unsaturated carbonyl compounds for cycloaddition reactions. These mild Lewis acids catalyze asymmetric 1,4-addition reactions of aryl thiols to enones with product selectivities up to 87% ee. P-31 NMR experiments provide an insight into the intricate equilibria governing the reaction mechanism. The absolute configuration of the major products indicates enones to react in the syn-s-trans orientation. Models based on X-ray structures of the Ru complexes can be used to rationalize selectivity.”
“HYPK (Huntingtin Yeast Partner K) was originally identified by yeast two-hybrid assay as an interactor of Huntingtin, the protein mutated in Huntington’s disease.

“
“Here, we report a numerical experiment in which submicrometer particle entrainment in a periodic flow that

matches those existing in the alveolus in the human lung was simulated for both sedentary and light activity. A spherical cavity with a prescribed velocity profile at the inlet was used to simulate the time-dependent periodical flow of air in the alveolus. Expansion and contraction of the alveolus were simulated by setting a conceptual permeable wall as the outer surface of the model and adjusting the boundary conditions in order to match the continuity of the flow. The simulations were conducted for breathing periods of 5 and GSK2126458 in vivo 3 s, which match sedentary and light activity conditions, respectively, and the results were extrapolated to the real lung. It was found that, most of the particles mainly followed a straightforward path and reached the opposite side of the alveolar wall in both breathing conditions. The concentration patterns obtained are consistent with the fact that the flow within the alveolus is mainly diffusive and does not greatly depend on the flow velocity. It was found that the particles which are heavier than air move out of phase with the periodic airflow that crosses the alveolus entrance, and that these particles are significantly caught within the alveolus. Particle entrapment increases with breathing rate

in accordance with experimental values and indicates that increase in breathing frequency in environments with high concentration of submicrometer particles has the consequence of increasing particle entrapment by several times with respect to normal breathing rate.”
“Age-related KU-57788 in vitro decline in microstructural integrity of certain white matter tracts may explain cognitive decline associated with normal

aging. Whole brain tractography and a clustering segmentation in 48 healthy individuals across the adult lifespan were used to examine: interhemispheric (corpus callosum), intrahemispheric association (cingulum, uncinate, arcuate, inferior longitudinal, inferior occipitofrontal), and projection (corticospinal) fibers. Principal components analysis reduced cognitive tests into 6 meaningful factors: (1) memory and executive function; (2) visuomotor dexterity; (3) motor LDK378 in vitro speed; (4) attention and working memory; (5) set-shifting/ flexibility; and (6) visuospatial construction. Using theory-based structural equation modeling, relationships among age, white matter tract integrity, and cognitive performance were investigated. Parsimonious model fit demonstrated relationships where decline in white matter integrity may explain age-related decline in cognitive performance: inferior longitudinal fasciculus (ILF) with visuomotor dexterity; the inferior occipitofrontal fasciculus with visuospatial construction; and posterior fibers (i. e., splenium) of the corpus callosum with memory and executive function.

The kinetics of benfuracarb sorption in mollisols conformed to two-compartment (1 + 1) first-order kinetics. The fast sorption rate constant was about 3 times higher for silt loam than for loam soil. However, the slow sorption rate constants were statistically similar for both soils. The concentration-dependent sorption-desorption isotherms of benfuracarb could not closely conform to the Freundlich isotherm in mollisols of high organic C content. MK-8931 The computed values of both the sorption (log K)

and desorption (log K’) capacities were higher for silt loam than for loam soil. The desorption index (n’/n) values in the range 30.0-41.3 indicated poor reversibility of sorbed benfuracarb in mollisols.\n\nCONCLUSION: In view of the strong sorption of benfuracarb in mollisols with only partial desorption, the possibility of the leaching of soil-applied benfuracarb to contaminate groundwaters appears to be low. (C) 2010 Society of Chemical Industry”
“PURPOSE: To compare the repeatability and reproducibility of ocular biometry and intraocular lens (IOL) power obtained with a new optical biometer (AL-Scan) and a standard optical biometer (IOLMaster 500). SETTING: Siriraj Hospital, Mahidol University, Bangkok, Thailand. DESIGN: Prospective comparative study. METHODS: Two independent operators measured

eyes with cataract using both biometers. The keratometry values, axial length, anterior chamber depth, white-to-white (WTW) corneal diameter, and IOL power calculated

using the Holladay PD-L1 inhibitor 1 formula obtained AZD6244 cost with each device were recorded. lntraoperator repeatability and interoperator reproducibility of both devices were analyzed using the intraclass correlation coefficient (ICC). The agreement in ocular biometry and IOL power between the 2 devices was evaluated by the Bland-Altman method. RESULTS: The study recruited 137 eyes of 81 patients. The repeatability and reproducibility of both devices were high for all ocular biometry measurements (ICC, 0.87-1.00). Except for the WTW corneal diameter (ICC, 0.44), the agreement between the biometers was also high (ICC, 0.98-0.99). The IOL powers calculated by the Holladay 1 formula were similar between the 2 biometers. CONCLUSION: The new optical biometer provided excellent repeatability and reproducibility for all ocular biometry. Agreement with the standard optical biometer was good except for the WTW corneal diameter. (C) 2014 ASCRS and ESCRS”
“A long-term photo-identification study of killer whales (Orcinus orca) in northern Norway was initiated in 1986, when their prey the Norwegian spring-spawning herring (Clupea harengus) started to winter in a complex fjord system. The aim of this work was to estimate population size and apparent survival rates in this killer whale population using photo-identification and mark-recapture techniques with data collected during October-December 1986 – 2003.

Although the expression of DRD3 in the ventral striatum has been shown to exhibit 24-hour variations, the mechanisms underlying the variation remain obscure. Here, we demonstrated that molecular components of the circadian clock act as regulators that control the 24-hour variation in the expression of DRD3. The transcription of DRD3 was enhanced by the retinoic acid-related orphan

receptor alpha (ROR alpha), and its activation was inhibited by the orphan receptor REV-ERB alpha, an endogenous antagonist of ROR alpha. The serum or dexamethasone-induced oscillation in the expression of DRD3 in cells was abrogated by the downregulation or overexpression of REV-ERB alpha, suggesting that REV-ERB alpha functions as a regulator of DRD3 oscillations in the cellular autonomous clock. Chromatin immunoprecipitation assays of the DRD3 promoter indicated that the binding of the REV-ERB alpha protein to the DRD3 promoter increased in the early dark phase. DRD3 protein selleck inhibitor expression varied with higher levels during the dark phase. Moreover, the effects of the DRD3 agonist 7-hydroxy-N, N-dipropyl-2-aminotetralin find more (7-OH-DPAT)-induced locomotor hypoactivity were significantly increased when DRD3 proteins were abundant. These results suggest that ROR alpha and REV-ERB alpha consist of a reciprocating mechanism wherein ROR alpha upregulates the expression of DRD3, whereas REV-ERB alpha periodically suppresses the expression at the

time of day when REV-ERB alpha is abundant. Our present findings revealed that a molecular link between the circadian clock and the function of DRD3 in the ventral striatum

acts as a modulator of the pharmacological actions of DRD3 agonists/antagonists.”
“In this review, the authors discuss the seemingly paradoxical loss of control associated with states of high readiness to execute a plan, termed VS-6063 molecular weight “intention-based reflexivity.” The review suggests that the neuro-cognitive systems involved in the preparation of novel plans are different than those involved in preparation of practiced plans (i.e., those that have been executed beforehand). When the plans are practiced, intention-based reflexivity depends on the prior availability of response codes in long-term memory (LTM). When the plans are novel, reflexivity is observed when the plan is pending and the goal has not yet been achieved. Intention-based reflexivity also depends on the availability of working-memory (WM) limited resources and the motivation to prepare. Reflexivity is probably related to the fact that, unlike reactive control (once a plan is prepared), proactive control tends to be relatively rigid”
“Purpose: To investigate a possible association between gene variants and patient response to treatment with intravitreal ranibizumab for neovascular age-related macular degeneration (AMD). Methods: Visual acuity score (VAS) was recorded at baseline and a subsequent visit at 6 months.

The findings of hypocellular neuropil as well as the characteristic ependymoblastic rosettes were seen. In addition the third case showed an abnormal combination of patterns including melanocytic and rhabdomyoblastic differentiation. The tumors stained positively for synaptophysin in the neuropil and small cell component, while the ependymoblastic rosettes stained for vimentin only. Epithelial membrane antigen and CD99 were negative in all components. One of the cases showed tetra-ploidy of chromosome 2. All cases exhibited an aggressive

course. This is a Galardin rare and recently recognized tumor with dismal outcome, and reporting of additional new cases should help in gaining more knowledge about it.”
“The aim of this study was to assess the effect of bifenthrin on common carp (Cyprinus carpio L.). The 96-h LC50 value of Talstar EC 10 (active substance 100 g l(-1) bifenthrin) was found to be 57.5 mu g l(-1). Examination of haematological and biochemical profiles and histological tissue examination was performed on common carp after 96 h of exposure to Talstar EC 10 (57.5 mu g l(-1)). The experimental group showed significantly higher (P < 0.01) values of plasma glucose, ammonia, aspartate aminotransferase and creatine kinase as well as the relative and absolute monocyte count, compared with the control group.

Histological examination revealed teleangioectasiae 3-MA molecular weight of secondary gill lamellae and degeneration of hepatocytes. The bifenthrin-based Talstar EC 10 pesticide preparation was classified as a substance strongly toxic for fish.”
“We performed molecular dynamics (MD) simulations of a Lennard-Jones system and investigated the effect of potential cutoff in the Nose-Poincare and Nose-Hoover thermostats. The Nose-Poincare thermostat is the symplectic algorithm of the Nose thermostat, while the Nose-Hoover thermostat is not a symplectic algorithm. If the potential energy is twice or more differentiable, the Hamiltonian

was conserved well in the Nose-Poincare thermostat. If the potential energy is once or less differentiable, however, the Hamiltonian was not conserved, but increased because the continuity of potential energy is required in a symplectic MD simulation. The increase in the Hamiltonian caused the increase in instantaneous temperature, and physical quantities cannot Selleck BMS-777607 be obtained correctly. It is because the difference in the Hamiltonian effectively increases the set temperature in the equations of motion. On the other hand, the Hamiltonian was not conserved for any cutoff method in the Nose-Hoover thermostat because it is not a symplectic algorithm. However, temperature was controlled appropriately because the Hamiltonian deviation does not affect the set temperature.”
“The novel species Halomonas zhaodongensis NEAU-ST10-25(T) recently identified by our group is a moderate halophile which can grow at the range of 0-2.5 M NaCl (optimum 0.5 M) and pH 6-12 (optimum pH 9).

In this study using computational analysis of sequenced rice genome, we identified eight and seven potential non-redundant members involved in AsA and tocochromanol biosynthetic pathways, respectively. Vorinostat mw The results reveal that the common feature

of these gene promoters is the combination of light-responsive, hormone-responsive, and stress-responsive elements. These findings, together with expression analysis in the MPSS database, indicate that AsA and 432 tocochromanols might be co-related with the complex signaling pathways involved in plant responses. (c) 2011 Elsevier Ltd. All rights reserved.”
“The cytotoxicity of polyelectrolytes commonly employed for layer-by-layer deposition of polyelectrolyte multilayers (PEMUs) was assessed using rat smooth muscle A7r5 and human osteosarcoma U-2 OS cells. Cell growth, viability, and metabolic assays were used to compare the responses

of both cell lines to poly(acrylic acid), PAA, and poly(allylamine hydrochloride), PAR, in solution at concentrations up to 10 mM and to varying thicknesses of (PAA/PAH) PEMUs. Cytotoxicity correlated with increasing concentration LY3039478 mouse of solution polyelectrolytes for both cell types and was greater for the positively charged PAR than for the negatively charged PAA. While metabolism and proliferation of both cell types was slower on PEMUs than on tissue culture plastic, little evidence for direct toxicity on cells was observed. In fact, evidence for more extensive adhesion and cytoskeletal organization was observed with PAR-terminated

PEMUs. Differences in cell activity and viability on different thickness PEMU surfaces resulted primarily from differences in attachment for these adhesion-dependent cell lines.”
“The human colon carcinoma cell line Caco-2 is often used as a model for intestinal drug absorption. To better understand xenobiotic glucuronidation in Caco-2 cells, we have examined the expression AZD7762 supplier levels of different UDP-glucuronosyltransferases (UGTs) in them. The effects of two main factors were investigated, namely, passage number and cell differentiation. Hence, the mRNA levels of 15 human UGTs of subfamilies 1A and 2B were assessed in both undifferentiated and fully differentiated cells at four passage levels: P31, P37, P43, and P49. Quantitative reverse transcriptase-polymerase chain reaction was used to determine the mRNA levels of individual UGTs, and the values were normalized using beta-actin as a reference gene. The results indicate that although passage number in the tested range exerts a mild effect on the expression level of several UGTs, the contribution of cell differentiation is much larger. The expression of nearly all the UGTs that were examined in this study was significantly, sometimes greatly, increased during cell differentiation.

Thus, chronic exposure of skin to UVB irradiation leads to histological changes consistent with aging, such as wrinkling, abnormal pigmentation, and loss of elasticity. We investigated the protective effect of the standardized green

tea seed extract (GSE) on UVB-induced skin photoaging in hairless mice. MATERIALS/METHODS: Skin photoaging was induced by UVB irradiation on the back of Skh-1 hairless mice three times per week and UVB irradiation was 432 performed for 10 weeks. Mice were divided into six groups; normal control, UVB irradiated control group, positive control (UVB + dietary supplement of vitamin C 100 SBC-115076 molecular weight mg/kg), GSE 10 mg/kg (UVB + dietary supplement of GSE 10 mg/kg), GSE 100 mg/kg (UVB + dietary supplement of GSE 100 mg/kg), and GSE 200 mg/kg (UVB + dietary supplement of GSE 200 mg/kg). RESULTS: The dietary supplement GSE attenuated UVB irradiation-induced wrinkle formation and the decrease in density of dermal collagen fiber. In addition, results of the antioxidant VX-680 analysis showed that GSE induced a significant increase in antioxidant enzyme activity compared with the UVB irradiation control group. Dietary supplementation with GSE 200 mg/kg resulted in a significant decrease in expression of MMP-1, MMP-3, and MMP-9 and an increase in expression of TIMP and type-1 collagen.

CONCLUSIONS: Findings of this study suggest CAL-101 nmr that dietary supplement GSE could be useful in attenuation of UVB irradiation-induced skin photoaging and wrinkle formation due to regulation of antioxidant defense systems and MMPs expression.”
“Background and Purpose An immature vascular phenotype in diabetes mellitus may cause more severe vascular damage and poorer functional outcomes after stroke, and it would be feasible to repair damaged functional vessels using endothelial progenitor cell (EPC) transplantation. However, high glucose induces p38 mitogen-activated protein kinase activation, which can accelerate the senescence

and apoptosis of EPCs. The aim of this study was to investigate the combined effects of EPC transplantation and p38 mitogen-activated protein kinase inhibitor administration on diabetic stroke outcomes. Methods Bone marrow-derived EPCs were injected intra-arterially into db/db mice after ischemic stroke induction. RWJ 67657 (RWJ), a p38 mitogen-activated protein kinase inhibitor, was administered orally for 7 consecutive days, with the first dose given 30 minutes before stroke induction. Functional outcome was determined at days 0, 1, 7, 14, and 21. Angiogenesis, neurogenesis, infarct volume, and Western blotting assays were performed on day 7, and white matter remodeling was determined on day 14. Results Neither EPC transplantation nor RWJ administration alone significantly improved diabetic stroke outcome although RWJ displayed a potent anti-inflammatory effect.

(C) 2013 Elsevier Ltd. All rights reserved.”
“Amniotic fluid embolism (AFE) is a rare complication of pregnancy, which, in France, is the second leading cause of maternal mortality. It results from the passage of amniotic fluid into the maternal circulation, but mechanisms

leading to clinical signs are not yet clearly identified. After a literature review, we report on the case of a 34 years old patient occurred during labor. Laboratory tests were performed during acute phase to diagnose coagulopathy and to monitor vital parameters. Further laboratory tests were conducted to support the diagnosis: cytology, study of insulin-like growth factor-binding protein 1, tryptase and alpha-foetoprotein. Performances and place of these laboratory tests must be precised as no specific test can attest

the diagnosis.”
“Background: A defective emotional enhancement of verbal memory (VM) performances has selleck chemicals been reported in different neurological diseases. Objectives: To assess the emotional enhancement of VM in 22 Clinically Isolated Syndrome (CIS) suggestive of Multiple 3 Sclerosis (MS) and 49 Relapsing (RR) MS patients in comparison to 32 Healthy Controls (HC). Methods: Immediate and delayed recall see more of VM was assessed using the Selective Reminding Test (SRT). A list of 12 emotionally significant words was used to evaluate the Emotional (E) variants of the SRT. Depressive symptoms were assessed by the Beck Depression Inventory. Results: The prevalence of depression did not differ between RRMS and CIS patients. Both patient groups showed poorer (p smaller than 0.01) VM performances in comparison to HC in all the SRT tasks, but no difference was found between the two patient groups. Emotionally salient words were more (p smaller than 0.0001) recalled than neutral words in HC and CIS, but not in RRMS patients, while performing BX-795 order the immediate recall tasks. Delayed recall was not

affected by emotional stimuli in both CIS and RR MS groups. The presence of depressive symptoms did not influence the VM performances. Conclusions: Our results demonstrate a defective emotional enhancement of VM in definite MS and, although to a lesser extent, in CIS patients. (C) 2014 Elsevier B.V. All rights reserved.”
“In the present study, we investigated the hepatoprotective effects of schisandrin B (Sch B) and green tea polyphenols (GrTP), two natural antioxidants, against Hg hepatotoxicity. Fifty-six rats were randomly divided into four groups of fourteen animals in each group: control group, Hg-treated group, Sch B pretreated group, and GrTP pretreated group. HgCl2 injection increased Hg accumulation, pathological and ultrastructural injury, lactate dehydrogenase (LDH) level, alanine transaminase (ALT) level, apoptosis, reactive oxygen species (ROS) level, glutathione (GSH) level, and malondialdehyde (MDA) level. HgCl2 injection decreased glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) activities.

(C) 2014 Elsevier Ltd. All rights reserved.”
“Historically, studies of brain metabolism have been based

on targeted analyses of a limited number of metabolites. Here we present an untargeted mass spectrometry-based metabolomic strategy that has successfully uncovered differences in a broad array of metabolites across anatomical regions of the mouse brain. The NSG immunodeficient mouse model was chosen because of its ability to undergo humanization leading to numerous applications in oncology and infectious disease research. Metabolic phenotyping by hydrophilic interaction liquid chromatography and nanostructure imaging mass spectrometry revealed both water-soluble and lipid metabolite patterns across brain regions. Neurochemical differences EVP4593 supplier in metabolic phenotypes were mainly defined by various phospholipids and several intriguing metabolites including carnosine, cholesterol sulfate, lipoamino acids, uric acid, and sialic acid, whose physiological roles in brain metabolism are poorly understood. This study helps define PR-171 in vivo regional homeostasis for the normal mouse brain to give context to the reaction

to pathological events.”
“Five new xanthenone O-glycosides, sibiricaxanthone C (1), sibiricaxanthone D (2), sibiricaxanthone E (3), sibiricaxanthone F (4), and sibiricaxanthone G (5) were isolated from the roots of Polygala sibirica L., together with the six known

xanthenone glycosides 6-11. The structures of new compounds were elucidated on the basis of spectral data and acid hydrolysis.”
“The phylogenetic relationships of notoungulates, an extinct group of predominantly South American herbivores, remain poorly resolved with respect to both other placental mammals and among one another. Most previous phylogenetic analyses of notoungulates have not included characters of the internal cranium, not least because few such features, including the bony labyrinth, have been described for members of the group. Here we describe the inner ears of the GDC-0941 molecular weight notoungulates Altitypotherium chucalensis (Mesotheriidae), Pachyrukhos moyani (Hegetotheriidae) and Cochilius sp. (Interatheriidae) based on reconstructions of bony labyrinths obtained from computed tomography imagery. Comparisons of the bony labyrinths of these taxa with the basally diverging notoungulate Notostylops murinus (Notostylopidae), an isolated petrosal from Itaborai, Brazil, referred to Notoungulata, and six therian outgroups, yielded an inner ear character matrix of 25 potentially phylogenetically informative characters, 14 of them novel to this study. Two equivocally optimized character states potentially support a pairing of Mesotheriidae and Hegetotheriidae, whereas four others may be diagnostic of Notoungulata.